The MRMAtlas is a compendium of targeted proteomics assays to detect and quantify yeast proteins in complex proteome digests by mass spectrometry. It results from high-quality measurements of yeast proteins conducted on a triple quadrupole mass spectrometer, and is intended as a resource for selected/multiple reaction monitoring (SRM/MRM)-based proteomic workflows.
The database at present is the largest resource of validated MRM assays of any organism. It currently contains assays for nearly 1500 S. cerevisiae proteins, corresponding to 21% of the yeast proteome and spanning all levels of abundance. Furthermore, it offers different software tools which allow users to browse through single proteins, through peptides and through cellular pathways. The optimal coordinates for establishing an MRM assay can be readily exported and uploaded to the SRM/MRM method of a triple quadrupole mass spectrometer. The assays can then be used to detect and quantify at high-throughput deliberately chosen set of target proteins in any biological sample of interest.